Objective To construct a medical assistant nursing staff management system. Methods Use EpiData3.1 software to input survey data, and use SPASS19.0 software for statistical analysis. Through patient care service satisfaction, nursing error rate, caregiver assessment pass rate, patient complaints, service defects, self-evaluation work quality, establishment of service quality sensitive indicators, etc. as the research objects, collect and sort out November and December 2018 Compare and analyze the data from June and July 2019. Results The average score of patient care service satisfaction increased from 88±91 to 93±98; the incidence of nursing errors decreased from 12 cases to 3 cases; the passing rate of nursing staff assessment increased from 72% to 92%; the complaints of patients rose from 1.2%± 2.12% The rate of decrease was 1.0%; the sensitive indicators of service quality increased from 7 to 14 items. Conclusion According to the "The Occupational Classification Code of the People's Republic of China (2015 Edition)", medical nurses are one of the auxiliary medical service personnel, mainly engaged in auxiliary nursing work. Under the new form of medical and health system reform, the construction of a medical assistant nursing staff management system can reflect the soft power of the hospital, enhance the core competitiveness of hospital services, and promote the sustainable and healthy development of the hospital.