microRNA-345在恶性胸膜间皮瘤细胞中的异常表达及其靶基因生物信息学分析

黄 何菲, 李 君贤, 彭 理, 范 岩鑫, 张 芳芳, 朱 丽瑾
杭州医学院公共卫生学院

摘要

目的:探究microRNA-345(miR-345)在恶性胸膜间皮瘤细胞中的异常表达,并利用生物信息学对其靶基因进行筛选及分析｡方法利用荧光定量PCR(qPCR)对恶性胸膜间皮瘤细胞(NCI-H2452､MSTO-211H､NCI-H2052)和正常间皮细胞MeT-5A细胞中miRNA-345表达水平进行检测分析,通过生物信息学分析预测miRNA-345下游基因,利用metascape分析miRNA-345下游基因的通路｡结果三株恶性间皮瘤细胞相比MeT-5A细胞miRNA-345的表达量均降低,生物信息学分析筛选出了26个miRNA-345下游基因(上调9个,下调17个),通过富集分析发现筛选出的miRNA-345下游基因主要参与脉管系统发育､细胞连接组织､上皮细胞迁移､Ras蛋白信号转导､细胞外基质组织和上皮细胞分化等信号通路｡结论miRNA-345在恶性胸膜间皮瘤细胞中表达量下降,生物信息学分析结果提示脉管系统发育､细胞连接组织､上皮细胞迁移､Ras蛋白信号转导､细胞外基质组织和上皮细胞分化等相关通路参与了miRNA-345在恶性胸膜间皮瘤细胞中的表达调控｡

关键词

恶性胸膜间皮瘤细胞;miRNA-345;生物信息学分析

参考

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