银杏双黄酮抑制NF-κB信号通路影响骨髓巨噬细胞向破骨细胞分化

吴 锋, 曾 艳平, 覃 万安, 林 舟丹, 程 昌志, 梁 雄波, 陈 子阳, 韦 葛堇
中国人民解放军联勤保障部队第九二三医院

摘要

目的本研究基于NF-κB通路探讨银杏双黄酮(ginkgetin,GK)对RANKL诱导的破骨细胞分化的影响及其作用机制｡方法采用不同浓度的GK(0､0.1､1､10和100µM)作用小鼠骨髓来源的巨噬细胞(BMMs)并分组,通过CCK8试剂检测各组的细胞增殖,TRAP染色检测破骨细胞生成能力,通过Western-blot检测IκBα蛋白表达｡结果高浓度GK(大于12.5µM)可显著抑制BMMs增殖,TRAP染色显示高浓度GK抑制破骨细胞形成,Western-blot结果显示,GK(50µM)组IκBα表达明显高于对照组｡结论在小鼠BMMs中,GK可抑制NF-κB信号通路进而影响RANKL诱导的破骨细胞分化｡

关键词

银杏双黄酮,NF-κB｡骨质疏松症,破骨细胞,RANKL

参考

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