真核起始因子6调控巨噬细胞迁移及抑制炎症因子

王 珍珍¹, 鲜 雪梅², 杨 帅², 李 欣², 陈 仁金²
1、徐州医科大学肿瘤生物治疗研究所
2、徐州医科大学生命科学学院

摘要

探索真核起始因子6(Eukaryoticinitiationfactor-6,eIF6)对巨噬细胞的影响｡eIF6慢病毒载体和空载体转染RAW264.7细胞,通过梯度嘌呤霉素筛选,获得eIF6过表达细胞和空载体对照组细胞｡荧光显微镜下检测细胞荧光表达;RealtimePCR检测细胞系中eIF6的mRNA表达及各炎症因子的表达｡细胞划痕实验检测eIF6过表达对巨噬细胞迁移的影响｡结果显示eIF6过表达巨噬细胞细胞的迁移速率显著降低于阴性对照组(P<0.05);eIF6过表达细胞中促炎标记物(iNOS,IL-1β,IL-4和MCP-1)表达显著升高(P<0.05),抗炎标记物(CD163,IL-10和TGF-β1)表达显著降低(P<0.05)｡eIF6调节巨噬细胞迁移及炎症因子的变化｡

关键词

真核起始因子6,巨噬细胞迁移,炎性因子

参考

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