生姜提取物6-姜酚调节炎症因子促进创面愈合的机制研究

侯 春, 王 肃生, 梁 刚, 张 志华, 冀 航, 王 平平, 施 然, 李 伯辉
广州医科大学附属第一医院

摘要

目的：探讨生姜提取物6-姜酚对创面愈合过程中炎症因子中性粒细胞、巨噬细胞如MPO、F4/80的影响及其机制。方法：利用C57BL/6小鼠并在其脊柱两侧各做一个直径8mm的全皮肤层切除的圆形创面模型，使用6-姜酚（GIN，30mg/kg/d）局部注射进行药物干预治疗。通过HE染色检测创面组织病理变化；通过免疫荧光检测创面中MPO、F4/80的表达变化；利用蛋白质免疫印迹技术和实时荧光定量技术检测创伤组织内炎症因子中MPO、F4/80的蛋白质和mRNA的表达情况。结果：术后第5天，HE染色显示6-姜酚治疗组的上皮间隙为(0.87±0.51)mm，模型组上皮间隙为(1.78±1.21)mm，结果比较显示有统计学意义（p=0.04）。免疫荧光染色中MPO阳性标记物中显示6-姜酚治疗组阳性细胞计数结果为(17.33±5.55)，模型组阳性细胞计数结果为(14.22±1.03)，结果比较显示无统计学意义（p=0.09）；F4/80阳性标记物中显示6-姜酚治疗组阳性细胞计数结果为（79.99±11.11），模型组阳性细胞计数结果为（71.23±1.88），结果比较显示有统计学意义（p=0.02）。6-姜酚治疗组、模型组MPOmRNA及蛋白表达水平分别为2.89±1.23、8.12±0.87和2.04±0.67、7.54±0.55(p=0.07，p=0.09)，6-姜酚治疗组、模型组F4/80mRNA及蛋白表达水平分别为3.23±0.56、6.26±3.76和2.76±0.23、3.01±1.23(p=0.02，p=0.02)。结论：6-姜酚可能通过调节炎症因子中的巨噬细胞表达进而促进小鼠创面愈合。

关键词

6-姜酚；MPO；F4/80；创伤愈合

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