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氧化石墨烯修饰肽纳米技术 对人体多能干细胞的增值抑制作用研究

王  舒, 陶  杨
重新市急救医疗中心

摘要


氧化石墨烯(Graphene oxide,GO)作为一种石墨烯的衍生物,能够影响细胞行为和分化,在干细胞治
疗方面发挥着非常重要的作用。为了实现诱导人多能干细胞(ihPSCs)在干细胞治疗中的治疗前景,本研究开发
出简单安全的ihPSCs增殖和成骨细胞谱系生物材料。构建了含氧化石墨烯(GO)的聚己内酯(PCL)修饰成骨肽
(BFP-1)微环境电纺纳米纤维支架,以改善ihPSC的体外细胞生长和成骨能力。使用CCK-8测定法评估ihPSCs的
粘附和增殖,结果表明ihPSCs能够有效增殖并保持无基质凝胶的成骨肽涂层纳米纤维的多能性。该研究结果在加速
ihPSCs在骨组织工程中的再生潜力方面具有相当大的应用前景。

关键词


氧化石墨烯;多能干细胞;增值抑制;研究

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参考


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DOI: http://dx.doi.org/10.12361/2705-0459-04-08-97765

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