氧化石墨烯修饰肽纳米技术 对人体多能干细胞的增值抑制作用研究

王 　舒, 陶 　杨
重新市急救医疗中心

摘要

氧化石墨烯（Graphene oxide，GO）作为一种石墨烯的衍生物，能够影响细胞行为和分化，在干细胞治
疗方面发挥着非常重要的作用。为了实现诱导人多能干细胞（ihPSCs）在干细胞治疗中的治疗前景，本研究开发
出简单安全的ihPSCs增殖和成骨细胞谱系生物材料。构建了含氧化石墨烯（GO）的聚己内酯（PCL）修饰成骨肽
（BFP-1）微环境电纺纳米纤维支架，以改善ihPSC的体外细胞生长和成骨能力。使用CCK-8测定法评估ihPSCs的
粘附和增殖，结果表明ihPSCs能够有效增殖并保持无基质凝胶的成骨肽涂层纳米纤维的多能性。该研究结果在加速
ihPSCs在骨组织工程中的再生潜力方面具有相当大的应用前景。

关键词

氧化石墨烯；多能干细胞；增值抑制；研究

参考

[1]S. Dobbenga, L.E. Fratila-Apachitei, A.A. Zadpoor,

Nanopattern-induced osteogenic differentiation of stem cells–A

systematic review, Acta biomaterialia 46 (2016) 3–14.

[2]V. Sottile, A. Thomson, J. McWhir, In vitro osteogenic

differentiation of human ES cells, Cloning & Stem Cells. 5 (2)

(2003) 149–155.

[3]A.P. Kishan, E.M. Cosgriff‐Hernandez, Recent

advancements in electrospinning design for tissue engineering

applications: a review, Journal of Biomedical Materials

Research Part A. 105 (10) (2017) 2892–2905.

[4]S. Wang, F. Hu, J. Li, S. Zhang, M. Shen, M. Huang,

et al., Design of electrospun nanofibrous mats for osteogenic

differentiation of mesenchymal stem cells, Nanomedicine:

Nanotechnology, Biology and Medicine. 14 (7) (2018) 2505–

2520.

[5]E. Saburi, M. Islami, S. Hosseinzadeh, A.S. Moghadam,

R.N. Mansour, E. Azadian, et al., In vitro osteogenic

differentiation potential of the human induced pluripotent

stem cells augments when grown on Graphene oxide-modified

nanofibers, Gene. 696 (2019) 72–79.

[6]A. Ardeshirylajimi, A. Khojasteh, Synergism of

electrospun nanofibers and pulsed electromagnetic field on

osteogenic differentiation of induced pluripotent stem cells,

Asaio Journal. 64 (2) (2018) 253–260.

[7]F.S. Hosseini, F. Soleimanifar, A. Khojasteh, A.

Ardeshirylajimi, Promoting osteogenic differentiation of

human‐induced pluripotent stem cells by releasing Wnt/

β‐catenin signaling activator from the nanofibers, Journal of

cellular biochemistry 120 (4) (2019) 6339–6346.