在人血小板中添加谷氨酰胺可防止紫外线C诱导的血小板活化的毒性作用

MazharMushtaq
苏莱曼·拉吉希大学基础医学系沙特阿拉伯

摘要

目的:血小板的主要功能是预防出血。输血血小板既可以预防,也可以治疗。从血液和血液成分的储存角度来看,世界各地都遵循标准协议,以确保血库的安全运行。利用紫外-c光处理血小板已成为血库保存血小板浓度的一种有价值的方法。然而,它的有害作用仍然存在,如激活血小板,从而失去其生理功能。本研究旨在证明在血小板浓缩液中添加谷氨酰胺可以预防UV-C的毒性作用。这项研究是用人类或小鼠的血小板进行的。所有实验均采用阳性对照和阴性对照,以确保实验结果的有效性。在血小板暴露于适当剂量的UV-C之前和之后评估体外血栓形成。为了评估50mM谷氨酰胺对血小板的细胞毒性作用,使用甲基噻唑四唑(MTT)来验证活力效应。我们已经成功地证明,使用谷氨酰胺可以减轻血小板浓缩物暴露于UV-C期间所造成的生理损害。我们的研究表明,添加适当浓度的谷氨酰胺可以保留UV-C对血小板的毒性作用。

关键词

血小板;谷氨酰胺;UV-C;血栓的形成

参考

[1]BashirS,CooksonP,WiltshireM,HawkinsL,SonodaL,ThomasS,etal.PathogeninactivationofplateletsusingultravioletClight:effectoninvitrofunctionandrecoveryandsurvivalofplatelets.Transfusion2013;53:990–1000.

[2]JohnsonL,HylandR,TanS,TolksdorfF,SumianC,SeltsamA,etal.InvitroqualityofplateletswithlowplasmacarryovertreatedwithultravioletClightforpathogeninactivation.TransfusionMed.Hemotherapy.2016;43:190–197.

[3]VanAelstB,DevlooR,VandekerckhoveP,CompernolleV,FeysHB.UltravioletClightpathogeninactivationtreatmentofplateletconcentratespreservesintegrinactivationbutaffectsthrombusformationkineticsoncollageninvitro.Transfusion2015;55:2404–2414.

[4]DeJagerTL,CockrellAE,DuPlessisSS.UltravioletLightInducedGenerationofReactiveOxygenSpecies.In:AhmadSI,editor.UltravioletLightinHumanHealth,DiseasesandEnvironment.Cham:SpringerInternationalPublishing2017;15–23.

[5]BrittVanAelst,RosalieDevloo,PhilippeVandekerckhove,etal.UltravioletClightpathogeninactivationtreatmentofplateletconcentratespreservesintegrinactivationbutaffectsthrombusformationkineticsoncollageninvitro.Transfusion2015;55:2404–2414.

[6]MurphyS,MunozS,Parry-BillingsM,NewsholmeE.Aminoacidmetabolismduringplateletstoragefortransfusion.BrJHaematol1992;81:585-590.

[7]MushtaqM,NamTS,KimUH.CriticalroleforCD38-mediatedCa2+signalinginthrombin-inducedprocoagulantactivityofmouseplateletsandhemostasis.JournalofBiologicalChemistry2011;15:12952–12958.

[8]Seltsam,Axel&Müller,Thomas.UVCIrradiationforPathogenReductionofPlateletConcentratesandPlasma.Transfusionmedicineandhemotherapy2011;38:43-54.

[9]MazharM,KimUH.TheRoleofGlutamineinthePreventionofUltraviolet-C-InducedPlateletActivation.BiochemistryResearchInternational.2020.DOI:8853696,7Pages.

[10]ShirleyOwusu-Ofori,JosephKusi,AlexOwusu-Ofori,GrahamFreimanis,etal.TreatmentofwholebloodwithriboflavinandUVlight:impactonmalariaparasiteviabilityandwholebloodstorage.Shock2014;44:33-38.

[11]JerroldH.L,MatthewD.Neal,andJayH.Herman.Bacterialcontaminationofplateletsfortransfusion:strategiesforprevention.CriticalCare2018;22:271-278.

[12]MohrH,SteilL,GravemannU,ThieleT,HammerE,GreinacherA,etal.Anovelapproachtopathogenreductioninplateletconcentratesusingshort-waveultravioletlight.Transfusion2009;49:2612–2624.

[13]SeltsamA,MullerTH.UVCirradiationforpathogenreductionofplateletconcentratesandplasma.TransfusionMed.Hemotherapy2011;38:43–54.

[14]FreimanisG.InvestigatingthePrevalenceofTransfusionTransmissionofPlasmodiumwithinaHyperendemicBloodDonationSystem.Transfusion2013;53:1429–1441.

[15]KabitaC,ShamsuzZ,RahulC,SurinderS,ShawnDK,ShaliniT,etal.EvaluationofMirasolpathogenreductionsystembyartificiallycontaminatingplateletconcentrateswithStaphylococcusepidermidis:ApilotstudyfromIndia.AsianJTransfusSci.2016;10:127–131.

[16]Asa’ahN,GabrielA,EmmanuelA,ClaudeT,TazoachaA.Wholebloodpathogenreductiontechnologyandbloodsafetyinsub_x005fSaharanAfrica:Asystematicreviewwithregionaldiscussion.AfrJLabMed.2016;5:363.

[17]TerpstraFGetal.PotentialandlimitationofUVCirradiationfortheinactivationofpathogensinplateletconcentrates.Transfusion2008;48:304-313.

[18]VandenBroekeT,DumontLJ,HunterS,NixonJ,MurphyS,RogerJ,etal.Plateletstoragesolutioneffectsontheaccuracyoflaboratorytestsforplateletfunction:amulti-laboratorystudy.VoxSanguinis2004;86:183-188.

[19]KarnickiK,JohnsonC,StCyrJ,EricsonD,RaoG.Plateletstoragesolutionimprovestheinvitrofunctionofpreservedplateletconcentrate.VoxSanguinis2003;85:262-266.

[20]ZhiL,ChiX,VostalJG.InvitroandinvivocharacterizationofultravioletlightC-irradiatedhumanplateletsina2eventmousemodeloftransfusion.PLoSOne2013;8:e79869.

[21]ValeriaV,ElisabettaM,MartaF,PaolaB.Glutaminetransportandenzymaticactivitiesinvolvedinglutaminolysisinhumanplatelets.BiochimBiophysActa1995;1243:43-48.

[22]ValeriaV,ElisabettaM,MartaF,PaolaB.GlutamineUtilizationinRestingandStimulatedPlatelets.TheJournalofBiochemistry1993;114:163–166.