化学发光免疫分析法在甲状腺疾病临床检验中的应用价值 Application value of chemiluminescence immunoassay in clinical examination of thyroid diseases

韩 晓利¹, Xiaoli Han²
1、河北省故城县医院
2、Hebei Gucheng County Hospital

摘要

目的：探讨在甲状腺疾病临床检验中应用化学发光免疫分析法的应用效果。方法：获得院伦理委员会准许和患者及其家属同意后，选取我院近两年收治的甲状腺患者64例，进行合理分组，展开详细调查，按照疾病类型将其划分为实验A组（甲状腺功能亢进）32例和实验B组（甲状腺肿瘤）32例，同时选取将我院32例健康体检的人员设为对照组，采用化学发光免疫分析法和放射免疫法这两种检验方式对所有人员进行检验。在整个检验过程中，需准确和详细记录下需要进行观察的指标（甲状腺球蛋白水平，符合率、特异度、敏感度），以便为后期数据研究提供数据支持。结果：根据研究数据可知，实验A组和实验B组患者的甲状腺球蛋白水平显著高于对照组，组间数据差异较大，P＜0.05；采用化学发光免疫法检测出实验A组（80.13±3.24）与实验B组（65.26±23.27）的甲状腺球蛋白水平较放射免疫法（50.26±2.93、48.29±23.82）更高，组间数据差异较大，P＜0.05；采用化学发光免疫法检测出的符合率、特异度、敏感度的数据分别为：实验A组（96.88%、90.63%、78.13%）、实验B组（93.75%、81.25%、81.25%），较放射免疫法更高，组间数据差异较大，P＜0.05。结论：就本次研究所得的具体数据做对比分析发现，在甲状腺疾病的临床检验中，需要采用化学发光免疫法来开展具体的检验工作，对提高临床检验效果帮助较大，为临床上对该疾病的诊断提供了有效依据。各项数据对比发现，采用该检验方式比之于放射免疫法具有明显的优势，因而，可推广。 Objective: To investigate the effect of chemiluminescence immunoassay (CLIA) in clinical examination of thyroid diseases.Methods:64 cases of thyroid patients admitted to our hospital in recent two years were divided into experimental group A (32 cases of hyperthyroidism) and experimental group B (32 cases of thyroid tumor) according to the type of disease.During the whole testing process, it is required to accurately and carefully record the indexes to be observed (thyroglobulin level, coincidence rate, specificity and sensitivity), so as to provide data support for later data research.Results: The levels of thyroglobulin in group A and group B were significantly higher than those in control group (P <0.05).The coincidence rate, specificity and sensitivity were 96.88%,90.63% and 78.13% in group A and 93.75%,81.25% and 81.25% in group B, respectively.Conclusion: The comparative analysis of the specific data obtained in this study shows that in the clinical test of thyroid diseases, chemiluminescence immunoassay is needed to carry out the specific test work, which is helpful to improve the clinical test effect and provides an effective basis for the clinical diagnosis of the disease.Compared with radioimmunoassay, this method has obvious advantages and can be popularized.

关键词

化学发光免疫分析法；甲状腺；临床检验 Chemiluminescence immunoassay; thyroid; clinical test

参考

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