宫颈癌淋巴脉管浸润的生物信息学分析

何 炫明
兰州市城关区甘肃中医药大小第一临床医学院甘肃兰州730000

摘要

目的: 本研究旨在探讨淋巴脉管浸润宫颈癌患者的潜在分子机制，筛选淋巴脉管浸润+的生物标志物。方法:采用差异分析和通路富集分析探索脉管浸润的分子机制。采用ssGSEA分析免疫浸润分布。将差异基因与免疫相关基因交集然后用单因素Cox回归筛选免疫预后基因，最后构建最优多变量COX模型。结果:宫颈癌脉管浸润阳性的差异基因富集在免疫相关通路。ssGSEA结果显示LVSI+患者的适应性免疫受到抑制。使用交集基因EREG和IL-9R构建Cox模型。

参考

[1]ZhouCF,MaJ,HuangL,etal.Cervicalsquamouscellcarcinoma-secretedexosomalmir-221-3ppromoteslymphangiogenesisandlymphaticmetastasisbytargetingvash1[J].Oncogene,2019,38(8):1256-1268.DOI:10.1038/s41388-018-0511-x[2]DingX,JiaX,WangC,etal.Adhx9-lncrna-mdm2interactionregulatescellinvasionandangiogenesisofcervicalcancer[J].CellDeathDiffer,2019,26(9):1750-1765.DOI:10.1038/s41418-018-0242-0[3]CrommeFV,AireyJ,HeemelsMT,etal.Lossoftransporterprotein,encodedbythetap-1gene,ishighlycorrelatedwithlossofhlaexpressionincervicalcarcinomas[J].JExpMed,1994,179(1):335-340.DOI:10.1084/jem.179.1.335[4]ShankaranV,IkedaH,BruceAT,etal.Ifngammaandlymphocytespreventprimarytumourdevelopmentandshapetumourimmunogenicity[J].Nature,2001,410(6832):1107-1111.DOI:10.1038/35074122[5]TianL,GoldsteinA,WangH,etal.Mutualregulationoftumourvesselnormalizationandimmunostimulatoryreprogramming[J].Nature,2017,544(7649):250-254.DOI:10.1038/nature21724[6]BabaI,ShirasawaS,IwamotoR,etal.Involvementofderegulatedepiregulinexpressionintumorigenesisinvivothroughactivatedki-rassignalingpathwayinhumancoloncancercells[J].CancerRes,2000,60(24):6886-6889[7]PurwarR,SchlapbachC,XiaoS,etal.Robusttumorimmunitytomelanomamediatedbyinterleukin-9-producingtcells[J].NatMed,2012,18(8):1248-1253.DOI:10.1038/nm.2856