RNAscope技术在细菌感染组织学研究的应用

毛 昕, 宋 旭东
华北理工大学 河北唐山 063000

摘要

目的：细菌性感染对人类健康的威胁越来越严重，逐渐受到广泛的重视。但细菌感染性疾病缺乏特异性临床表现及影像学改变而难以确诊，细菌培养对标本要求高且耗时耗力，病理学改变主要以肉芽肿性改变为主，难以明确菌种类型，影响患者进一步治疗。RNAscope技术是一项新型的原位杂交技术，在不破坏组织学的基础上对病变进行观察，具有较高的敏感性及特异性。本文概述近年来RNAscope技术在细菌感染组织学诊断的应用。

关键词

RNAscope技术；细菌感染；组织学

参考

[1]黄晶晶,肖盟,徐英春等.二代测序技术在微生物和感染性疾病中的应用[J].协和医学杂志,2018,9(5):448-452.[2]王荣山,吴亦栋,尚世强.实时荧光定量PCR检测细菌方法的建立及其临床应用[J].中华围产医学杂志,2005,8(4): 242-245.[3] Gross-Thebing T,Paksa A,Raz E.Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos[J].BMC Biol,2014,12:55.[4]Wang F,Flanagan J, Su N et al.RNAscope:a novel in situ RNA analysis platform for formalin-fixed,paraffin-embedded tissues[J].J Mol Diagn,2012,14(1):22-29.[5]Baltzarsen PB,Georgsen JB,Nielsen PS et al.Detection of mRNA of Telomerase Protein in Benign Naevi and Melanomas Using RNAscope[J].Appl Immunohistochem Mol Morphol,2020,28(1):36-41.[6]Amini Chermahini G,Rashnonejad A,Harper SQ.RNAscope in situ hybridization-based method for detecting DUX4 RNA expression in vitro[J].Rna,2019,25(9):1211-1217.[7]汤鸿,朱颖炜,陆忠华.RNAscope技术在肝脏疾病组织学研究应用中的价值分析[J].中华肝脏病杂志,2020,(04):365-368.[8]杨海玉,刘勇.RNAscope 原位杂交技术在肿瘤病理学中的应用[J].中华病理学杂志,2017,46(4):283-285.[9]Bullman S,Pedamallu CS,Sicinska E et al.Analysis of Fusobacterium persistence and antibiotic response in colorectal cancer[J]. Science, 2017,358(6369): 1443-1448.[10]Liang M,Liu Y,Zhang Z et al.Fusobacterium nucleatum induces MDSCs enrichment via activation the NLRP3 inflammosome in ESCC cells,leading to cisplatin resistance[J].Ann Med, 2022,54(1):989-1003.[11]Gelbard A,Katsantonis NG,Mizuta M et al.Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species[J].Laryngoscope,2017,127(1):179-185.[12]Bonifacio JPP, Schmolke M.Visualization of Respiratory Commensal Bacteria in Context of Their Natural Host Environment[J].Front Microbiol,2021,12:678389.[13]Shimbori C,De Palma G,Baerg L et al.Gut bacteria interact directly with colonic mast cells in a humanized mouse model of IBS[J]. Gut Microbes,2022,14(1):2105095.[14]Palmer MV,Wiarda J,Kanipe C et al.Early Pulmonary Lesions in Cattle Infected via Aerosolized Mycobacterium bovis[J].Vet Pathol,2019,56(4):544-554.[15]Palmer MV,Thacker TC,Waters W.R.Differential Cytokine Gene Expression in Granulomas from Lungs and Lymph Nodes of Cattle Experimentally Infected with Aerosolized Mycobacterium bovis[J].PLoS One,2016,11(11):e0167471.[16]Palmer MV,Thacker TC,Waters WR.Multinucleated giant cell cytokine expression in pulmonary granulomas of cattle experimentally infected with Mycobacterium bovis[J].Vet Immunol Immunopathol,2016,180:34-39.[17]Popovics P.,Jain A.,Skalitzky K.O.et al.Osteopontin Deficiency Ameliorates Prostatic Fibrosis and Inflammation[J].Int J Mol Sci,2021,22(22):12461.