REST表达与胶质瘤卒中患者预后相关性研究

刘 坤, 屈 祥勇, 邹 礼
湖南省脑科医院（湖南省第二人民医院）神经外科 湖南长沙 410007

摘要

目的：探讨 REST 表达与胶质瘤卒中患者预后相关性研究。方法：收集患者的年龄、性别、体重、身高、民族、既往史、家族肿瘤史、手术时间、手术类型、肿瘤大小、肿瘤出血量、WHO 分期，检测对 21 例胶质瘤卒中患者肿瘤组织和 10 例脑外伤正常脑组织样本中GFAP、Ki67、P53、IDH1 突变、MGMT 启动子突变，分析 REST 的表达水平。结果：REST 的高表达与患者的肿瘤大小，肿瘤出血量、WHO分期以及 P53、Ki67 显著性相关。同时 REST 高表达患者预后相比 REST 低表达患者总体生存期更短。结论：REST 表达水平与胶质瘤卒中患者术后预后正相关，为神经胶质瘤卒中患者的治疗提供新理论依据。

关键词

关键词：神经元限制性沉默因子，胶质瘤，卒中，预后

参考

[1]《中国中枢神经系统胶质瘤诊断和治疗指南》编写组. 中国中枢神经系统胶质瘤诊断和治疗指南(2012 精简版)[J]. 中华医学杂志,2012,92(33): 2309-2313. [2]Omuro A, DeAngelis LM. Glioblastoma and Other Malignant Gliomas: A Clinical Review[J]. Journal of the American Medical Association,2013,310(17):1842-50. [2]郭烈美, 邱永明, 葛建伟, 等. 高级别胶质瘤术中肿瘤卒中的可能机制及治疗策略. 中国微侵袭神经外科杂志. 2012. 17(11): 505-506. [4]Li H,Liu Z,Wu Y, et al. The deficiency of NRSF/REST enhances the pro-inflammatory function of astrocytes in a model of Parkinson's disease[J]. Biochim Biophys Acta Mol Basis Dis,2020,1866(1): 165590. [5] Kim M, Ryu KY. Regulation of REST levels overcomes dysregulation of neural stem cell differentiation caused by disruption of polyubiquitin gene Ubb[J]. Biochem Biophys Res Commun,2017,486(1): 171-177. [6] Navarrete-Modesto V, Orozco-Suárez S, Alonso-Vanegas M, et al. REST/NRSF transcription factor is overexpressed in hippocampus of patients with drug-resistant mesial temporal lobe epilepsy[J]. Epilepsy Behav,2019,94: 118-123. [7]Kurita J, Hirao Y, Nakano H, et al. Sertraline, chlorprothixene, and chlorpromazine characteristically interact with the REST-binding site of the corepressor mSin3, showing medulloblastoma cell growth inhibitory activities[J]. Scientific reports,2018,8(1):13763. [8]Blom T, Tynninen O, Puputti M, et al. Molecular genetic analysis of the REST/NRSF gene in nervous system tumors[J]. Acta neuropathologica,2006,112(4):483-90. [9]Conti L, Crisafulli L, Caldera V, et al. REST controls self-renewal and tumorigenic competence of human glioblastoma cells[J]. PloS one,2012,7(6):e38486. [10]Bergsland M, Covacu R, Perez Estrada C, et al. Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells[J]. Stem cells (Dayton, Ohio),2014,32(9):2539-49. [11]Marisetty A, Lu L, Veo B, et al. REST-DRD2 mechanism impacts glioblastoma stem cell-mediated tumorigenesis[J]. Neuro-oncology,2019,21(6):775-85. [12] Kamal M, Sathyan P, Singh S, et al. REST regulates oncogenic properties of glioblastoma stem cells[J]. Stem cells,2012,30(3):405-14. [13]Wang E, Sandberg R, Luo S, et al. Alternative isoform regulation in human tissue transcriptomes[J]. Nature,2008,456(7221):470-6. [14]Wang R, Degirmenci V, Xin H, et al. PEI-Coated Fe₃O₄ Nanoparticles Enable Efficient Delivery of Therapeutic siRNA Targeting REST into Glioblastoma Cells[J].International journal of molecular sciences,2018,19(8). 本课题由湖南省科技厅自然科学基金项目（2021JJ70088）资助