金荞麦中药制剂对LPS诱导急性肺部炎症的治疗作用研究

黄 双, 游 佳豪, 陈 淑培, 郭 一璇, 曾 利, 李弘 德*
长沙医学院 湖南长沙 410219

摘要

摘要：目的：探索金荞麦中药制剂对脂多糖（lipopolysaccharide，LPS）诱导急性肺炎的治疗作用和初步机制。方法：将40只SPF级6～8周龄的雌性BALB/C小鼠按体重随机分为5组，即正常组、LPS诱导急性肺炎模型组（模型组）、金荞麦中药制剂-威麦宁胶囊低剂量治疗组（100mg/Kg体重）、中剂量治疗组（200mg/Kg体重）、高剂量治疗组（300mg/Kg体重），每组8只。对各治疗组小鼠灌胃给药1周，一天一次，对正常组和模型组小鼠灌胃等容量生理盐水。末次给药1h后，除正常组外其余各组采用鼻吸入法对小鼠施加100μl的1g/LLPS诱导急性肺炎，建模6h后处死小鼠并取材。采用ELISA实验检测小鼠支气管肺泡灌洗液和肺组织匀浆中炎症因子白细胞介素-1β（interleukin-1β，IL-1β）、肿瘤坏死因子-α（tumornecrosisfactor-α，TNF-α）的水平，HE染色观察肺组织病理学变化，借助免疫组织化学法检测肺组织IL-1β和TNF-α的表达。结果：与模型组相比，不同剂量的威麦宁治疗均可改善小鼠肺间质水肿，减少中性粒细胞等炎症细胞渗出，减轻肺泡壁增厚程度，同时降低支气管肺泡灌洗液、肺组织匀浆和肺组织中IL-1β、TNF-α的水平。其中，中剂量治疗组对肺组织损伤改善效果最佳，对炎症因子IL-1β和TNF-α的抑制作用最明显。结论：动物实验表明，金荞麦中药制剂对LPS诱导的急性肺炎模型具有显著治疗作用，其机制可能与抑制肺组织中炎症因子IL-1β和TNF-α的水平有关。

关键词

关键词：金荞麦；威麦宁胶囊；脂多糖；急性肺炎；炎症因子

参考

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