负载BMP-2的自放电PVDF/BT纳米纤维支架促进成骨分化

吴 锋锋, 金 明超, 陈 卓, 王 国荣, 徐 艳
浙江省湖州市中心医院骨科 浙江湖州 313000

摘要

摘要：目的：通过静电纺丝技术制备负载BMP-2的PVDF/BT纳米支架，并评价其促成骨分化性能。方法：利用静电纺丝技术制备PVDF/BT纳米纺丝支架，随后使用多巴胺涂布其表面，用于负载BMP-2，以实现BMP-2的缓释。以PVDF/BT纳米纺丝支架为对照组，用扫描电镜(Scanning electron microscope，SEM)对纳米纺丝支架进行形貌表征，用ELISA分析BMP-2的负载率和累计释放情况，最后用SEM、CCK-8、PCR等方法评估成骨细胞MC3T3-E1在支架上的增殖分化情况。结果：制备的静电纺丝支架在涂布多巴胺之后直径较PVDF/BT支架普遍更大，表面更粗糙，说明多巴胺成功涂布于静电纺丝支架上；ELISA结果表明，涂布多巴胺之后，支架对BMP-2的负载率高达71%，且负载在支架上的BMP-2可持续释放长达28天。SEM图提示负载BMP-2的自放电PVDF/BT纳米纤维支架(PDB)上成骨细胞生长状态较纯PVDF/BT纳米纤维(P)更好，CCK-8结果也提示其细胞活性更高。PCR结果表明PDB组成骨细胞ALP、COL-I、OCN和RUNX2等成骨基因表达较P组明显升高，提示PDB支架可促进成骨分化。

关键词

关键词：成骨分化；压电效应；静电纺丝纤维；多巴胺；BMP-2

参考

[1]Graham, S. M., Leonidou, A., et al. 2010 Biological therapy of bone defects: the immunology of bone allo-transplantation Expert Opin Biol Ther,10 (6):885-901.[2]Wang,X.Yu,J.et al.2016 Electrospun nanofibrous materials: a versatile medium for effective oil/water separation Materials Today,19(7):403-414.[3]Shamos, M. H., Lavine, L. S., et al. 1963 Piezoelectric effect in bone Nature,197:81.[4]Damaraju,S.M.Shen,Y.et al.2017 Three-dimensional piezoelectric fibrous scaffolds selectively promote mesenchymal stem cell differentiation Biomaterials,149:51-62.[5]Fernandes, M. M., Correia, D. M., et al. 2019 Bioinspired Three-Dimensional Magnetoactive Scaffolds for Bone Tissue Engineering ACS Appl Mater Interfaces,11 (48):45265-45275.[6]Gregorio, R. and Cestari, M. 1994 EFFECT OF CRYSTALLIZATION TEMPERATURE ON THE CRYSTALLINE PHASE CONTENT AND MORPHOLOGY OF POLY(VINYLIDENE FLUOR IDE) Journal of Polymer Science Part B-Polymer Physics,32 (5):859-870.[7]Sencadas, V., Gregorio, R., et al. 2009 α to β Phase Transfor mation and Microestructural Changes of PVDF Films Induced by Uniaxial Stretch Journal of Macromolecular Science, Part B,48 (3):514-525.[8]Shepelin, N. A., Glushenkov, A. M., et al. 2019 New developpments in composites, copolymer technologies and processing techniques for flexible fluoropolymer piezoelectric generators for efficient energy harvesting Energy & Environmental Science,12 (4):1143-1176.[9]Bouhamed, A., Binyu, Q., et al. 2021 A hybrid piezoelectric composite flexible film based on PVDF-HFP for boosting power generation Composites Science and Technology,208.[10]Ongun, M. Z., Oguzlar, S., et al. 2020 Enhancement of piezoelectric energy-harvesting capacity of electrospun β-PVDF nanogenerators by adding GO and rGO Journal of Materials Science-Materials in Electronics,31 (3):1960-1968.[11]Wu,H.Dong,H.et al. 2023 Electrical stimulation of piezoelectric BaTiO3 coated Ti6Al4V scaffolds promotes anti-inflammatory polarization of macrophages and bone repair via MAPK/JNK inhibition and OXPHOS activation Biomaterials,293:121990.[12]Lo,K.W,Ulery,B.D,et al.2012 Studies of bone morphogenetic protein-based surgical repair Adv Drug Deliv Rev,64 (12):1277-1291.[13]Elias,E.Nasser,Z., et al. 2018 Bone Morphogenetic Protein Usage in Anterior Lumbar Interbody Fusion: What Else Can Go Wrong? World Neurosurg,111:55-59.