过表达VEGF和bFGF的骨髓间充质干细胞促进了大鼠缺血皮瓣的血管新生及存活

竺 枫¹, 殷 杰², 祝 斌², 滕 晓峰², 杨 科跃²
1、通讯作者宁波市第六医院
2、宁波市第六医院

摘要

摘要：目的：本研究旨在探索共过表达VEGF和bFGF的BMSCs对大鼠缺血皮瓣血管新生与存活的影响。方法：经脂质体介导的VEGF和bFGF共转染后，评估BMSCs对HUVEC增殖的作用；建立SD大鼠缺血皮瓣模型，设PBS、BMSC、BMSC+VEGF+bFGF(BVbF)组，进行对应处理并检测VEGF、bFGF、eNOS表达及皮瓣存活率、血流与毛细血管密度。结果：BVbF组较其他组更有效促进HUVEC增殖、皮瓣存活、血流恢复及eNOS的表达。结论：VEGF和bFGF共表达BMSCs有助于缺血皮瓣血管新生和存活，可能通过增强eNOS表达发挥作用。

关键词

关键词：血管内皮生长因子；碱性成纤维生长因子；骨髓间充质干细胞；缺血皮瓣；内皮型一氧化氮合酶

参考

[1]Tang X, Ren, J, Wei X, et al. Exploiting synergistic effect of CO/NO gases for soft tissue transplantation using a hydrogel patch[J].Nat Commun 2023, 14(1): 2417.[2]Afrooghe A, Damavandi AR, Ahmadi E, et al. The current state of knowledge on how to improve skin flap survival: A review[J]. J Plast Reconstr Aesthet Surg, 2023, 82:48-57.[3]Tang YH, Pennington LA, Scordino JW, et al. Dynamics of early stem cell recruitment in skin flaps subjected to ischemia reperfusion injury[J]. Pathophysiology. 2016, 23(3):221-228.[4]Pei YK, Zhang LC, Mao XY, et al. Biomaterial scaffolds for improving vascularization during skin flap regeneration[J]. Chinese Journal of Plastic and Reconstructive Surgery, 2020, 2(2): 109-119.[5]Hsueh YY, Wang DH, Huang TC, et al. Novel skin chamber for rat ischemic flap studies in regenerative wound repair[J]. Stem Cell Res Ther. 2016, 7(1):72.[6]徐永清, 何晓清. 皮瓣外壳的新进展[J]. 中国修复重建外科杂志, 2018, 32(7): 781-785.[7]Wang X, Li C, Zheng Y, et al. Bone marrow mesenchymal stem cells increase skin regeneration efficiency in skin and soft tissue expansion[J]. Expert Opin Biol Ther. 2012, 12(9):1129-1139.[8]Spanholtz TA, Theodorou P, Holzbach T, et al. Vascular endothelial growth factor (VEGF165) plus basic fibroblast growth factor (bFGF) producing cells induce a mature and stable vascular network--a future therapy for ischemically challenged tissue[J].J Surg Res.2011,171(1):329-338.[9]Lu GD, Cheng P, Liu T, Wang Z. BMSC-derived exosomal miR-29a promotes angiogenesis and osteogenesis[J]. Front Cell Dev Biol. 2020, 8:608521.[10]Dulak J, Guzik TJ. Angiogenesis, stem cells, eNOS and inflammation--the many faces of vascular biology[J]. Thromb Haemost. 2012, 108(5):801-803.[11]Yang X, Li X, Luo M, et al. Tubeimoside I promotes angiogenesis via activation of eNOS-VEGF signaling pathway[J]. J Ethnopharmacol. 2021, 267:113642.[12]Grochot-Przeczek A, Dulak J, Jozkowicz A. Therapeutic angiogenesis for revascularization in peripheral artery disease[J]. Gene. 2013, 525(2):220-228.