不同极化类型巨噬细胞外泌体对大鼠放射损伤骨髓间充质干细胞作用的初步研究

张维 文1¹, 宗春 琳2¹, 唐子 豪1¹, 赵 路2¹, 田 磊2¹, 关 键1²
1、1.佳木斯大学口腔医院2.军事口腔医学国家重点实验室，口腔疾病国家临床医学研究中心，陕西省口腔疾病临床医学研究中心，第四军医大学口腔医院口腔颌面外科
2、通讯作者1.佳木斯大学口腔医院2.军事口腔医学国家重点实验室，口腔疾病国家临床医学研究中心，陕西省口腔疾病临床医学研究中心，第四军医大学口腔医院口腔颌面外科

摘要

目的探索不同极化类型巨噬细胞来源外泌体对放射损伤后的骨髓间充质干细胞的作用。方法培养并鉴定大鼠骨髓来源巨噬细胞（BMDMs）并诱导为不同极化类型（M1、M2型），收集不同极化类型巨噬细胞来源的外泌体（M1-exo、M2-exo），培养鉴定骨髓间充质干细胞（BMSCs）并分为对照组、放射组（irBMSCs）、M1-exo组（irBMSCs+M1-exo）、M2-exo组（irBMSCs+M2-exo）。采用CCK-8及Ki-67染色检测各组细胞增殖能力、TUNNEL染色检测各组细胞凋亡情况、茜素红染色检测各组成骨分化能力、Westernblot检测ALP，RUNX2，a-SMA的表达情况。结果放射组BMSCs与对照组相比，增殖能力显著下降、TUNNEL阳性细胞数显著增多、成骨分化能力显著下降、RUNX2及ALP表达显著减低、α-SMA表达显著升高；M2-exo组与放射组相比，增殖能力显著增高、TUNNEL阳性细胞数显著减少、成骨分化能力显著增高、RUNX2及ALP表达显著增加、α-SMA表达显著降低。结论X射线造成BMSCs细胞损伤、成骨分化能力受损，M2型巨噬细胞来源的外泌体能够显著改善放射造成的BMSCs损伤、提高成骨分化能力、降低纤维化分化能力。

关键词

骨髓间充质干细胞（BMSCs）；放射损伤；巨噬细胞；外泌体；放射性颌骨骨坏死

参考

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