适应性实验室发展为生物发现和工业生物技术的有效工具

赫尔 弗特, 安德 鲁·, 加布 里奥, 阿尔 伯特
University of Alberta

摘要

因为培养技术、DNA测序、生物信息学和基因工程的进展有所提升，对于利用自然选择的过程来了解与掌
握新的微生物表型的可能性有很大的帮助。因此，适应性实验室进化（ALE）实验可算是一种很强大的研究，既可
以探讨影响菌株表型、性能和稳定性的进化力量，又可以获得包含有益突变的生产菌株。在这篇综述中，我们通过
收集强调进化可以促进菌株构建过程的多种方式的案例研究，总结和分类ALE在与工业生物生产相关的微生物生理
学的各个方面的应用。此外，本文还探讨了为实验设计提供信息的原则、协助高效使用计算建模等候补方式，以及
透过被采用并持续优化在自动化领域里，在未来以ALE作为一种高效应变设计与构建工具。

关键词

自适应实验室进化；突变体；生物技术；基因型；代谢

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